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anti human ccl22 neutralizing antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti human ccl22 neutralizing antibody
    Anti Human Ccl22 Neutralizing Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ccl22+antibody/Human+CCL22%2FMDC+Antibody/pm42815071-107-31-35
    Average 93 stars, based on 12 article reviews
    anti human ccl22 neutralizing antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Injection:

    Article Title: Aflatoxin B1 and Epstein-Barr virus-induced CCL22 expression stimulates B cell infection.
    Article Snippet: .. Briefly, CCL22 function was neutralized by treating the mice with a neutralizing anti- CCL22 antibody (abCCL22; mouse IgG2B anti human ccl22 R&D system clone 57226 lot BIK0316091) at 20 μg per injection per mouse every week. ..

    Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin, or esophageal injury
    Article Snippet: .. Seven-week-old Trail –/– female mice received a single thoracic x-ray irradiation dose of 20 Gy with shielding of other organs and were treated with either 20 μg of anti-CCL22 antibody (AF439, R&D Systems Inc.) dissolved in 500 μL of sterile normal saline via i.p. injection every other day for 2 weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems Inc.) dissolved in 500 μL of normal saline via i.p. injection every other day for 2 weeks ( n = 5 per treatment per group). ..

    Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin or esophageal injury
    Article Snippet: .. 7 week old Trail-/- female mice received a single thoracic x-ray irradiation dose of 20-Gy with shielding of other organs and were either treated with 20 μg of anti-CCL22 antibody (AF439, R&D Systems) dissolved in 500 μL of sterile normal saline via IP injection every other day for two- weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems) dissolved in 500 μL of normal saline via IP injection every other day for two-weeks (n = 5/treatment/group). ..

    Irradiation:

    Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin, or esophageal injury
    Article Snippet: .. Seven-week-old Trail –/– female mice received a single thoracic x-ray irradiation dose of 20 Gy with shielding of other organs and were treated with either 20 μg of anti-CCL22 antibody (AF439, R&D Systems Inc.) dissolved in 500 μL of sterile normal saline via i.p. injection every other day for 2 weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems Inc.) dissolved in 500 μL of normal saline via i.p. injection every other day for 2 weeks ( n = 5 per treatment per group). ..

    Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin or esophageal injury
    Article Snippet: .. 7 week old Trail-/- female mice received a single thoracic x-ray irradiation dose of 20-Gy with shielding of other organs and were either treated with 20 μg of anti-CCL22 antibody (AF439, R&D Systems) dissolved in 500 μL of sterile normal saline via IP injection every other day for two- weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems) dissolved in 500 μL of normal saline via IP injection every other day for two-weeks (n = 5/treatment/group). ..

    Sterility:

    Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin, or esophageal injury
    Article Snippet: .. Seven-week-old Trail –/– female mice received a single thoracic x-ray irradiation dose of 20 Gy with shielding of other organs and were treated with either 20 μg of anti-CCL22 antibody (AF439, R&D Systems Inc.) dissolved in 500 μL of sterile normal saline via i.p. injection every other day for 2 weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems Inc.) dissolved in 500 μL of normal saline via i.p. injection every other day for 2 weeks ( n = 5 per treatment per group). ..

    Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin or esophageal injury
    Article Snippet: .. 7 week old Trail-/- female mice received a single thoracic x-ray irradiation dose of 20-Gy with shielding of other organs and were either treated with 20 μg of anti-CCL22 antibody (AF439, R&D Systems) dissolved in 500 μL of sterile normal saline via IP injection every other day for two- weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems) dissolved in 500 μL of normal saline via IP injection every other day for two-weeks (n = 5/treatment/group). ..

    Saline:

    Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin, or esophageal injury
    Article Snippet: .. Seven-week-old Trail –/– female mice received a single thoracic x-ray irradiation dose of 20 Gy with shielding of other organs and were treated with either 20 μg of anti-CCL22 antibody (AF439, R&D Systems Inc.) dissolved in 500 μL of sterile normal saline via i.p. injection every other day for 2 weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems Inc.) dissolved in 500 μL of normal saline via i.p. injection every other day for 2 weeks ( n = 5 per treatment per group). ..

    Article Title: TRAIL agonists rescue mice from radiation-induced lung, skin or esophageal injury
    Article Snippet: .. 7 week old Trail-/- female mice received a single thoracic x-ray irradiation dose of 20-Gy with shielding of other organs and were either treated with 20 μg of anti-CCL22 antibody (AF439, R&D Systems) dissolved in 500 μL of sterile normal saline via IP injection every other day for two- weeks or 20 μg of polyclonal goat IgG (AB-108-C, R&D Systems) dissolved in 500 μL of normal saline via IP injection every other day for two-weeks (n = 5/treatment/group). ..

    Recombinant:

    Article Title: H3K18 lactylation in cancer-associated fibroblasts drives malignant pleural effusion progression via TNFR2 + T reg recruitment
    Article Snippet: .. In specific experimental conditions, the following reagents were added to the lower chamber: anti-CCL17 antibody (50 ng/ml, #MAB364-SP, R&D Systems), anti-CCL20 antibody (50 ng/ml, #MAB360-SP, R&D Systems), anti-CCL22 antibody (50 ng/ml, #MAB336-SP, R&D Systems), anti-CXCL16 antibody (50 ng/ml, #MAB976, R&D Systems) or recombinant human CXCL16 (100 ng/ml, #976-CX-025/CF, R&D Systems). ..

    Article Title: H3K18 lactylation in cancer-associated fibroblasts drives malignant pleural effusion progression via TNFR2 + T reg recruitment.
    Article Snippet: .. In specific experimental conditions, the following reagents were added to the lower chamber: anti-CCL17 antibody (50 ng/ml, #MAB364-SP, R&D Systems), antiCCL20 antibody (50 ng/ml, #MAB360-SP, R&D Systems), anti-CCL22 antibody (50 ng/ml, #MAB336-SP, R&D Systems), anti-CXCL16 antibody (50 ng/ ml, #MAB976, R&D Systems) or recombinant human CXCL16 (100 ng/ml, #976-CX-025/CF, R&D Systems). ..

    Neutralization:

    Article Title: PGE 2 -EP2/EP4 signaling elicits immunosuppression by driving the mregDC-Treg axis in inflammatory tumor microenvironment.
    Article Snippet: For CD8 T cells depletion in WTmice treated with vehicle or EP2 KOmice treated EP4 antagonists, IgG2b isotype (Clone: LTF-2, BioXCell) or anti-CD8a antibody (Clone: 2.43, BioXCell) were injected intraperitoneally at days 1, 0, 3 at the dose of 100 mg, and thereafter at days 7 and 14 at the dose of 200 mg. .. For Ccl17 and Ccl22 neutralization in vivo, anti-Ccl17 antibody and anti-Ccl22 antibody (goat anti-mouse antibodies; R&D Systems) were administered i.p. at 20 mg/mouse on alternate days starting from day 10 after tumor transplantation. .. Normal goat IgG (R&D) was used as isotype control.

    In Vivo:

    Article Title: PGE 2 -EP2/EP4 signaling elicits immunosuppression by driving the mregDC-Treg axis in inflammatory tumor microenvironment.
    Article Snippet: For CD8 T cells depletion in WTmice treated with vehicle or EP2 KOmice treated EP4 antagonists, IgG2b isotype (Clone: LTF-2, BioXCell) or anti-CD8a antibody (Clone: 2.43, BioXCell) were injected intraperitoneally at days 1, 0, 3 at the dose of 100 mg, and thereafter at days 7 and 14 at the dose of 200 mg. .. For Ccl17 and Ccl22 neutralization in vivo, anti-Ccl17 antibody and anti-Ccl22 antibody (goat anti-mouse antibodies; R&D Systems) were administered i.p. at 20 mg/mouse on alternate days starting from day 10 after tumor transplantation. .. Normal goat IgG (R&D) was used as isotype control.

    Transplantation Assay:

    Article Title: PGE 2 -EP2/EP4 signaling elicits immunosuppression by driving the mregDC-Treg axis in inflammatory tumor microenvironment.
    Article Snippet: For CD8 T cells depletion in WTmice treated with vehicle or EP2 KOmice treated EP4 antagonists, IgG2b isotype (Clone: LTF-2, BioXCell) or anti-CD8a antibody (Clone: 2.43, BioXCell) were injected intraperitoneally at days 1, 0, 3 at the dose of 100 mg, and thereafter at days 7 and 14 at the dose of 200 mg. .. For Ccl17 and Ccl22 neutralization in vivo, anti-Ccl17 antibody and anti-Ccl22 antibody (goat anti-mouse antibodies; R&D Systems) were administered i.p. at 20 mg/mouse on alternate days starting from day 10 after tumor transplantation. .. Normal goat IgG (R&D) was used as isotype control.

    Isolation:

    Article Title: Prolactin enhances T regulatory cell promotion of breast cancer through the long form prolactin receptor
    Article Snippet: .. Ten million splenocytes, isolated from Foxp3 + EGFP Balb/c mice were placed in the upper chamber (5 μm pore size, Corning 3421) and 1 mL conditioned medium from 4T1 cells with or without prolactin treatment was added to the lower chamber in the presence or absence of 1 μg/mL mouse anti-CCL17 or anti-CCL22 antibody (R & D systems, AF529, AF479, respectively). ..

    Pore Size:

    Article Title: Prolactin enhances T regulatory cell promotion of breast cancer through the long form prolactin receptor
    Article Snippet: .. Ten million splenocytes, isolated from Foxp3 + EGFP Balb/c mice were placed in the upper chamber (5 μm pore size, Corning 3421) and 1 mL conditioned medium from 4T1 cells with or without prolactin treatment was added to the lower chamber in the presence or absence of 1 μg/mL mouse anti-CCL17 or anti-CCL22 antibody (R & D systems, AF529, AF479, respectively). ..



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    a KEGG pathway enrichment of DEGs between FACS-sorted TNFR2 + and TNFR2 − CD4 + T cells from MPE ( n = 3). b , c Volcano plot ( b ) and heatmap ( c ) of DEGs related to the chemokine signaling pathway. d , e Flow cytometry histograms ( d ) and comparisons of chemokine receptor expression (CXCR6, CCR4 and CCR6) ( e ) on TNFR2 + T reg cells and TNFR2 − T reg cells ( n = 13). f Schematic of Transwell chemotaxis assay testing TNFR2 − T reg chemotaxis toward MPE supernatant (by Figdraw). g , h Transwell chemotaxis assay comparing TNFR2 + T reg frequencies between freshly isolated cells and cells that migrated toward MPE supernatant after 4 h incubation. i Concentrations of CXCL16, CCL17, <t>CCL22</t> and CCL20 in MPE and PB were quantified using ELISA ( n = 16). j Schematic of chemotaxis assay to investigate the chemotatic axis to attract TNFR2 + T reg cells in MPE. k , l Flow cytometry histograms and comparisons of chemotaxis of TNFR2 + T reg cells in response to MPE in the presence of anti-CXCL16, anti-CCL17, anti-CCL22 or anti-CCL20 mAbs. Data shown in d , e , g – i , k and l are representative of at least three independent experiments (mean ± s.d.). Statistical analysis was performed using paired two-tailed Student’s t -test ( e and h ), Wilcoxon test ( i ) or one-way ANOVA ( l ). * P < 0.05, ** P < 0.01, **** P < 0.0001. ns not significant, mAbs monoclonal antibodies.
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    a KEGG pathway enrichment of DEGs between FACS-sorted TNFR2 + and TNFR2 − CD4 + T cells from MPE ( n = 3). b , c Volcano plot ( b ) and heatmap ( c ) of DEGs related to the chemokine signaling pathway. d , e Flow cytometry histograms ( d ) and comparisons of chemokine receptor expression (CXCR6, CCR4 and CCR6) ( e ) on TNFR2 + T reg cells and TNFR2 − T reg cells ( n = 13). f Schematic of Transwell chemotaxis assay testing TNFR2 − T reg chemotaxis toward MPE supernatant (by Figdraw). g , h Transwell chemotaxis assay comparing TNFR2 + T reg frequencies between freshly isolated cells and cells that migrated toward MPE supernatant after 4 h incubation. i Concentrations of CXCL16, CCL17, <t>CCL22</t> and CCL20 in MPE and PB were quantified using ELISA ( n = 16). j Schematic of chemotaxis assay to investigate the chemotatic axis to attract TNFR2 + T reg cells in MPE. k , l Flow cytometry histograms and comparisons of chemotaxis of TNFR2 + T reg cells in response to MPE in the presence of anti-CXCL16, anti-CCL17, anti-CCL22 or anti-CCL20 mAbs. Data shown in d , e , g – i , k and l are representative of at least three independent experiments (mean ± s.d.). Statistical analysis was performed using paired two-tailed Student’s t -test ( e and h ), Wilcoxon test ( i ) or one-way ANOVA ( l ). * P < 0.05, ** P < 0.01, **** P < 0.0001. ns not significant, mAbs monoclonal antibodies.
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    R&D Systems human anti ccl22 antibody
    a KEGG pathway enrichment of DEGs between FACS-sorted TNFR2 + and TNFR2 − CD4 + T cells from MPE ( n = 3). b , c Volcano plot ( b ) and heatmap ( c ) of DEGs related to the chemokine signaling pathway. d , e Flow cytometry histograms ( d ) and comparisons of chemokine receptor expression (CXCR6, CCR4 and CCR6) ( e ) on TNFR2 + T reg cells and TNFR2 − T reg cells ( n = 13). f Schematic of Transwell chemotaxis assay testing TNFR2 − T reg chemotaxis toward MPE supernatant (by Figdraw). g , h Transwell chemotaxis assay comparing TNFR2 + T reg frequencies between freshly isolated cells and cells that migrated toward MPE supernatant after 4 h incubation. i Concentrations of CXCL16, CCL17, <t>CCL22</t> and CCL20 in MPE and PB were quantified using ELISA ( n = 16). j Schematic of chemotaxis assay to investigate the chemotatic axis to attract TNFR2 + T reg cells in MPE. k , l Flow cytometry histograms and comparisons of chemotaxis of TNFR2 + T reg cells in response to MPE in the presence of anti-CXCL16, anti-CCL17, anti-CCL22 or anti-CCL20 mAbs. Data shown in d , e , g – i , k and l are representative of at least three independent experiments (mean ± s.d.). Statistical analysis was performed using paired two-tailed Student’s t -test ( e and h ), Wilcoxon test ( i ) or one-way ANOVA ( l ). * P < 0.05, ** P < 0.01, **** P < 0.0001. ns not significant, mAbs monoclonal antibodies.
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    Image Search Results


    a KEGG pathway enrichment of DEGs between FACS-sorted TNFR2 + and TNFR2 − CD4 + T cells from MPE ( n = 3). b , c Volcano plot ( b ) and heatmap ( c ) of DEGs related to the chemokine signaling pathway. d , e Flow cytometry histograms ( d ) and comparisons of chemokine receptor expression (CXCR6, CCR4 and CCR6) ( e ) on TNFR2 + T reg cells and TNFR2 − T reg cells ( n = 13). f Schematic of Transwell chemotaxis assay testing TNFR2 − T reg chemotaxis toward MPE supernatant (by Figdraw). g , h Transwell chemotaxis assay comparing TNFR2 + T reg frequencies between freshly isolated cells and cells that migrated toward MPE supernatant after 4 h incubation. i Concentrations of CXCL16, CCL17, CCL22 and CCL20 in MPE and PB were quantified using ELISA ( n = 16). j Schematic of chemotaxis assay to investigate the chemotatic axis to attract TNFR2 + T reg cells in MPE. k , l Flow cytometry histograms and comparisons of chemotaxis of TNFR2 + T reg cells in response to MPE in the presence of anti-CXCL16, anti-CCL17, anti-CCL22 or anti-CCL20 mAbs. Data shown in d , e , g – i , k and l are representative of at least three independent experiments (mean ± s.d.). Statistical analysis was performed using paired two-tailed Student’s t -test ( e and h ), Wilcoxon test ( i ) or one-way ANOVA ( l ). * P < 0.05, ** P < 0.01, **** P < 0.0001. ns not significant, mAbs monoclonal antibodies.

    Journal: Experimental & Molecular Medicine

    Article Title: H3K18 lactylation in cancer-associated fibroblasts drives malignant pleural effusion progression via TNFR2 + T reg recruitment

    doi: 10.1038/s12276-025-01557-3

    Figure Lengend Snippet: a KEGG pathway enrichment of DEGs between FACS-sorted TNFR2 + and TNFR2 − CD4 + T cells from MPE ( n = 3). b , c Volcano plot ( b ) and heatmap ( c ) of DEGs related to the chemokine signaling pathway. d , e Flow cytometry histograms ( d ) and comparisons of chemokine receptor expression (CXCR6, CCR4 and CCR6) ( e ) on TNFR2 + T reg cells and TNFR2 − T reg cells ( n = 13). f Schematic of Transwell chemotaxis assay testing TNFR2 − T reg chemotaxis toward MPE supernatant (by Figdraw). g , h Transwell chemotaxis assay comparing TNFR2 + T reg frequencies between freshly isolated cells and cells that migrated toward MPE supernatant after 4 h incubation. i Concentrations of CXCL16, CCL17, CCL22 and CCL20 in MPE and PB were quantified using ELISA ( n = 16). j Schematic of chemotaxis assay to investigate the chemotatic axis to attract TNFR2 + T reg cells in MPE. k , l Flow cytometry histograms and comparisons of chemotaxis of TNFR2 + T reg cells in response to MPE in the presence of anti-CXCL16, anti-CCL17, anti-CCL22 or anti-CCL20 mAbs. Data shown in d , e , g – i , k and l are representative of at least three independent experiments (mean ± s.d.). Statistical analysis was performed using paired two-tailed Student’s t -test ( e and h ), Wilcoxon test ( i ) or one-way ANOVA ( l ). * P < 0.05, ** P < 0.01, **** P < 0.0001. ns not significant, mAbs monoclonal antibodies.

    Article Snippet: In specific experimental conditions, the following reagents were added to the lower chamber: anti-CCL17 antibody (50 ng/ml, #MAB364-SP, R&D Systems), anti-CCL20 antibody (50 ng/ml, #MAB360-SP, R&D Systems), anti-CCL22 antibody (50 ng/ml, #MAB336-SP, R&D Systems), anti-CXCL16 antibody (50 ng/ml, #MAB976, R&D Systems) or recombinant human CXCL16 (100 ng/ml, #976-CX-025/CF, R&D Systems).

    Techniques: Flow Cytometry, Expressing, Chemotaxis Assay, Isolation, Incubation, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Bioprocessing